human gbm cell line u251  (ATCC)

Journal: Experimental and Therapeutic Medicine

Article Title: Peimine induces apoptosis of glioblastoma cells through regulation of the PI3K/AKT signaling pathway

doi: 10.3892/etm.2024.12737

Figure Lengend Snippet: Effects of peimine on the cell viability and colony formation of glioblastoma cells. (A) The chemical structure of peimine. (B) HEB, (C) U87 and (D) U251 cells were treated with different doses of peimine (0, 6.25, 12.5, 25, 50 and 100 µM) for 24, 48, and 72 h. An MTT assay was used to determine the viability of the cells. (E) Colony formation assays were performed using U87 cells, (F) which were quantified. Data are presented as the mean ± SD from three repeats. Data were analyzed using a one-way ANOVA followed by a Tukey's post-hoc test. ** P<0.01 and **** P<0.0001 vs. the control.

Article Snippet: Procell Life Science & Technology Co., Ltd. provided the human GBM cell lines U87 (cat. no. CL-0238) and U251 (cat. no. CL-0237), in addition the normal human brain glial cell line HEB (cat. no. CL0130).

Techniques: MTT Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Peimine induces apoptosis of glioblastoma cells through regulation of the PI3K/AKT signaling pathway

doi: 10.3892/etm.2024.12737

Figure Lengend Snippet: Peimine reduces migration and invasion of glioblastoma cells. (A) Wound healing assays were performed using U87 cells treated with peimine. Scale bar, 500 µm. (B) Quantification of wound healing assay. (C) Transwell assays were used to assess migration and invasion of U87 cells. Scale bar, 100 µm. Quantification of (D) migration and (E) invasion assays. Data are presented as the mean ± SD from three repeats. Data were analyzed using a one-way ANOVA followed by a Tukey's post-hoc test. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control.

Article Snippet: Procell Life Science & Technology Co., Ltd. provided the human GBM cell lines U87 (cat. no. CL-0238) and U251 (cat. no. CL-0237), in addition the normal human brain glial cell line HEB (cat. no. CL0130).

Techniques: Migration, Wound Healing Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Peimine induces apoptosis of glioblastoma cells through regulation of the PI3K/AKT signaling pathway

doi: 10.3892/etm.2024.12737

Figure Lengend Snippet: Peimine induces apoptosis in glioblastoma cells. (A) Following the treatment of U87 cells with varying doses of peimine, the ROS levels were measured. (B) Semiquantification of (A). (C) After peimine treatment for 24 h, the mitochondrial membrane potential of stained U87 cells was examined using a fluorescence microscope. (D) Apoptosis changes in stained U87 cells after peimine treatment for 24 h, performed by Annexin V-FITC/PI double staining. Scale bar, 100 µm. (E) Semi-quantification of (D). Data are presented as the mean ± SD of three repeats. Data were analyzed using a one-way ANOVA followed by a Tukey's post-hoc test. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control. ROS, reactive oxygen species.

Article Snippet: Procell Life Science & Technology Co., Ltd. provided the human GBM cell lines U87 (cat. no. CL-0238) and U251 (cat. no. CL-0237), in addition the normal human brain glial cell line HEB (cat. no. CL0130).

Techniques: Membrane, Staining, Fluorescence, Microscopy, Double Staining, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Peimine induces apoptosis of glioblastoma cells through regulation of the PI3K/AKT signaling pathway

doi: 10.3892/etm.2024.12737

Figure Lengend Snippet: Peimine induces apoptosis in GBM cells through regulation of the PI3K/AKT signaling pathway. (A) Western blot analysis of p53, Bax, Bcl-2, Caspase 3 and Cleaved-Caspase 3 expression in peimine-treated U87 cells. (B) Semi-quantifcation of (A). (C) Western blot analysis of PI3K, p-PI3K, AKT and p-AKT protein levels in peimine-treated U87 cells. (D) Semi-quantification of (C). Cells were pretreated with 0 and 25 µM peimine (E) with or without SC79 pretreatment for 1 h or (F) with or without MK2206 pretreatment for 24 h, before viability was evaluated using an MTT assay. Data were analyzed using a one-way ANOVA followed by a Tukey's post-hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001 vs. control. p-, phosphorylated.

Article Snippet: Procell Life Science & Technology Co., Ltd. provided the human GBM cell lines U87 (cat. no. CL-0238) and U251 (cat. no. CL-0237), in addition the normal human brain glial cell line HEB (cat. no. CL0130).

Techniques: Western Blot, Expressing, MTT Assay, Control